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aec buffer  (Vector Laboratories)


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    Structured Review

    Vector Laboratories aec buffer
    Aec Buffer, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1307 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/substrate+buffer+aec/AEC+Peroxidase+(HRP)+Substrate+Kit%2C+3-amino-9-ethylcarbazole/pmc11561467-409-3-9
    Average 96 stars, based on 1307 article reviews
    aec buffer - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Application of mechanical-force sensitive macrophage subset in pancreatic cancer diagnosis or prognosis evaluation
    Article Snippet: .. Each slide was incubated with 100 μL of CD8 primary antibody diluent (1:100; Cat. No.853365, CST) at room temperature for 1 hour, and washed with TBST for three times after incubation, 2 minutes each time; added with HistoFine secondary antibody (Vector, Mouse/Rabbit) according to the resistance of the primary antibody to incubate for 30 minutes; and then added with an AEC color-developing solution (Cat. No. SK-4200, Vector lab, 10 mL ddH2O+1st Reagent 4 Drops+2st Reagent 6 Drops+3st Reagent 4 Drops) prepared, and wrapped with a tin foil to store in the dark at 4° C. 4) After incubation, the slides were shaken and washed with TBST for three times, 2 minutes each time, and then transferred into deionized water for simple cleaning. ..

    Article Title: Antidiabetic Effects of Quercetin and Silk Sericin in Attenuating Dysregulation of Hepatic Gluconeogenesis in Diabetic Rats Through Potential Modulation of PI3K/Akt/FOXO1 Signaling: In Vivo and In Silico Studies
    Article Snippet: Afterward, the samples were retrieved by heating in 1× target retrieval solution (pH 6.0, DAKO, Glostrup, Denmark), followed by blocking with a peroxidase-blocking solution for 15 min. After washing with PBS buffer, the hepatic sections were blocked with serum block solution for 10 min prior to being incubated overnight at 4 °C with the primary antibody caspase-3 (1:100, D175, Cell Signaling Technology, Beverly, MA, USA). .. Following washing with PBS buffer, the hepatic sections were treated with AEC (3-Amino-9-ethylcarbazole) substrate chromogens, followed by incubation with a horseradish peroxidase (HRP)-labeled micropolymer conjugated with secondary antibody (MP-7451, anti-rabbit IgG; Vector Labs, Burlingame, CA, USA) for 1 h [ ]. .. Subsequently, the slides were washed, stained with hematoxylin as a secondary stain, and then probed employing a light microscope (Olympus CX31, Tokyo, Japan).

    Article Title: Ixodes ricinus bites promote allergic skin inflammation and intestinal tuft and mast cell expansion in mice
    Article Snippet: Slides were then blocked with rabbit serum (Vector Laboratories) and incubated with rat-anti-mouse MBP (provided by Dr. Elizabeth Jacobsen, Mayo Clinic, Rochester, USA). .. After incubation with biotinylated rabbit anti-rat IgG, followed by AB complex (Vector Laboratories), staining was finally visualized with AEC+ high sensitivity substrate chromogen solution (Vector Laboratories) and counterstained with hematoxylin. ..

    Staining:

    Article Title: Ixodes ricinus bites promote allergic skin inflammation and intestinal tuft and mast cell expansion in mice
    Article Snippet: Slides were then blocked with rabbit serum (Vector Laboratories) and incubated with rat-anti-mouse MBP (provided by Dr. Elizabeth Jacobsen, Mayo Clinic, Rochester, USA). .. After incubation with biotinylated rabbit anti-rat IgG, followed by AB complex (Vector Laboratories), staining was finally visualized with AEC+ high sensitivity substrate chromogen solution (Vector Laboratories) and counterstained with hematoxylin. ..



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